• Volume/Page
  • Keyword
  • DOI
  • Citation
  • Advanced
   
 
 
 

Journal of the Acoustical Society of America

SECTION LISTING

BROWSE VOLUMES

Year Range: 
Search Issue | RSS Feeds RSS
Previous Issue Next Issue

Nov 1980

Volume 68, Issue S1, pp. S1-S116

Return to All Sections
back to top
RSS Feeds
back to top Session II. Physiological Acoustics IV: Neurochemistry of the Auditory System
Invited Papers
FREE

Immunohistochemical visualization of transmitter related substances in the cochlea (A)

Jorgen Fex

J. Acoust. Soc. Am. Volume 68, Issue S1, pp. S64-S64 (1980); (1 page)

Online Publication Date: 11 Aug 2005

Full Text: | Download PDF

Show Abstract
Immunofluorescence and horseradish peroxidase indirect markers for antibodies have been used to study neurotransmitter‐related substances in the cochlea of the guinea pig and cat. General aspects of the methods will be discussed and specific examples presented.
FREE

Identification of the auditory nerve neurotransmitter (A)

Robert J. Wenthold

J. Acoust. Soc. Am. Volume 68, Issue S1, pp. S64-S64 (1980); (1 page)

Online Publication Date: 11 Aug 2005

Full Text: | Download PDF

Show Abstract
Several lines of evidence suggest that glutamate or aspartate is a neurotransmitter for the auditory nerve. Both amino acids are concentrated in terminals of the auditory nerve and two enzymes associated with the metabolism of glutamate and aspartate, glutaminase and aspartate aminotransferase, are present in terminals and fibers of the auditory nerve. Glutamate and aspartate are released from slices of the cochlear nucleus and their release is decreased by lesion of the auditory nerve. The auditory nerve neurotransmitter is pharmacologically similar to glutamate and aspartate. Immunocytochemical localization of aspartate aminotransferase in the cochlear nucleus will also be discussed.
FREE

Microchemical mapping studies of neurotransmitter chemistry in the cochlear nucleus (A)

Donald A. Godfrey

J. Acoust. Soc. Am. Volume 68, Issue S1, pp. S64-S64 (1980); (1 page)

Online Publication Date: 11 Aug 2005

Full Text: | Download PDF

Show Abstract
Quantitative histochemical procedures [O. H. Lowry and J. V. Passonneau, A Flexible System of Enzymatic Analysis (Academic, New York, 1972), pp. 219–260] have been applied together with a mapping approach to study the distributions of neurotransmitter systems in the cochlear nucleus. The methods involve quick‐freezing of tissue, followed by frozen sectioning, freeze‐drying, dissection of sections with microknives, weighing of samples on microbalances, and direct chemical assays in microliter volumes. Structures as small as 20 μM per edge can be chemically analyzed by these methods, and the histological locations of all samples are recorded, allowing direct comparisons with anatomical and physiological data for the same locations. The distributions of proposed transmitter amino acids and of the enzymes of acetylcholine metabolism have been mapped in the cochlear nuclei of rats and cats. The effects on these distributions of transections of anatomical pathways are being studied. Results so far suggest that, in the rat cochlear nucleus, most cholinergic synapses may be related to centrifugal, or feedback, pathways to the nucleus.
FREE

Light and electron microscopic autoradiographic approaches to the chemistry of auditory brain stem nuclei (A)

Ilsa R. Schwartz

J. Acoust. Soc. Am. Volume 68, Issue S1, pp. S65-S65 (1980); (1 page)

Online Publication Date: 11 Aug 2005

Full Text: | Download PDF

Show Abstract
A fresh brain slice preparation has been developed which allows the simultaneous characterization of the anatomical localization of radioactively labeled substances in a variety of different auditory brain stem areas. Labeled substances may include putative neurotransraittcrs, ligands for receptor binding sites and antibodies to specific proteins. Varying incubation conditions can be applied to adjacent slices, avoiding the complication of differences between non‐inbred animals. These slices can be examined at both the light and electron microscopic level. Differential distributions of label in cells and synaptic terminal populations in the cat cochlear nucleus following incubations with putative neurotransmitter tritiated amino acids suggest differences in the chemical properties of at least three classes of terminals in the dorsal cochlear nucleus. Studies are underway to describe the differential labeling patterns in several auditory nuclear groups and to characterize the conditions under which they occur. [Work supported by NIH.]
Return to All Sections
Close

Home Publications Meetings Contact ASA Site Map Back to Top

Copyright © Acoustical Society of America

close