The enrichment of the neon isotopes in a thermoacoustic device is demonstrated. Because the thermal diffusion ratio of neon is small, an apparatus longer than a wavelength was necessary in order to easily observe the separation. The device was modular and extensible, so that arbitrarily large separations could in principle be obtained. The acoustic duct was a series of multiple, identical quarter-wavelength modules with side-branch drivers. In this way, waveforms close to that of a traveling wave were maintained in the duct, despite the high acoustic attenuation caused by the duct’s small diameter and large length. The concentrations of the isotopes were measured at one end of the duct using a quadrupole mass spectrometer. For the operating frequency of 227 Hz, the maximum separation gradient obtained was 0.43%/m, and mole fluxes at zero gradient as high as 3 nmol/s were observed. Effects of turbulence, though not observed, are also discussed, and the scaling properties of this method are compared with those of traditional mixture-separation methods. © 2004 Acoustical Society of America.